Molecular characterization and genetic diversity of Staphylococcus aureus isolates of dairy production farms in Rio de Janeiro, Brazil

Staphylococcus aureus is an important pathogen involved in subclinical bovine mastitis, causing high economic losses for the dairy industry. The successful persistence of this host occurs due to a series factors associated with colonization ability and acquisition virulence factors. This bacterial species carries genetic heterogeneity, genetically diverse strains characterize population. Analysis variation tool epidemiological studies. For study, S. were randomly selected by molecular profiling. All originated from milk cows mastitis on farms State Rio de Janeiro. Strains profiled using gene analysis profiles, agr spa typing, Pulsed Field Gel Electrophoresis (PFGE), Multilocus Sequence Typing (MLST). 47% (8/17) tested positive icaA gene; 82.3% (14/17) icaD 41% (7/17) fbnA fbnB 94% (16/17) hlA 70.5% (12/17) hlB gene. These results generated 11 different profiles. Most (58.8% - 10/17) classified as type-II system. typing identified seven types. PFGE found extensive heterogeneity no clones observed. MLST five types ST/CC. Considering observed at present variety presence justified absence clonal properties evaluated. Besides, that, definition strain well dynamic population not fully understood since there are limited number studies mastitis.